Evaluation of Plasma Levels of Interferon-Gamma, Interleukin-6, and Transforming Growth Factor-Beta in Under-Five Children with Malaria Parasitaemia
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Abstract
BACKGROUND: The interaction between the pro- and anti-inflammatory cytokines is known to play key roles in the immune response to infectious diseases. The pathogenesis of malaria parasitemia, including its progression to symptomatic manifestation, also seems to be strongly related to this interplay.
AIM: The study evaluated the plasma levels of interferon-gamma (IFN-γ) and interleukin-6 (IL)-6, which are pro- inflammatory cytokines, and transforming growth factor-beta (TGF-β), which is an anti-inflammatory cytokine; in the 6–60 months age-group children, when infected with Plasmodium falciparum, to show their relevance in the development of immunity against malaria.
METHODS: The study was a cross-sectional study involving children with uncomplicated malaria parasitemia. In the study, malaria parasitemia was confirmed by microscopy, using the Giemsa stain. The enzyme-linked immunosorbent assay (ELISA) method was used to evaluate the plasma levels of IFN-γ, IL-6, and TGF-β in the under-five children infected with P. falciparum, and their counterparts who were not infected with the parasite.
RESULTS: The median plasma IFN-γ, IL-6, and TGF-β levels in participants with malaria parasitemia were 225.15 pg/ mL, 123.31 pg/mL, and 2091.02 pg/mL, respectively. The difference in the plasma levels of TGF-β in the infected and uninfected participants was statistically significant with a p < 0.001.
CONCLUSION: The findings in this work showed that malaria parasitemia in under-five children is associated with significant depression in the plasma level of TGF-β when compared to their uninfected counterparts.
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References
World Health Organization. World Malaria Report 2022. Geneva: World Health Organization; 2022. Available from: https://www.who.int/teams/global-malaria-program [Last accessed on 2023 Jan 25].
Lalremruata A, Jeyaraj S, Engleitner T, Joanny F, Lang A, Belard S, et al. Species and genotype diversity of Plasmodium in malaria patients from Gabon analysed by next generation sequencing. Malar J. 2017;16(1):398. https://doi.org/10.1186/s12936-017-2044-0 PMid:28974215
World Health Organization. Guidelines for Malaria. Geneva: World Health Organization; 2022. Available from: https://apps.who.int/iris/handle/10665/351995 [Last accessed on 2023 Jan 25].
Awosolu OB, Yahaya ZS, Haziqah MT, Simon-Oke IA, Fakunle C. A cross-sectional study of the prevalence, density, and risk factors associated with malaria transmission in urban communities of Ibadan, Southwestern Nigeria. Heliyon. 2021;7(1):e05975. https://doi.org/10.1016/j.heliyon.2021.e05975 PMid:33521357
White M, Watson J. Age, exposure and immunity. Elife. 2018;7:e40150. https://doi.org/10.7554/elife.40150 PMid:30129437
Sato S. Plasmodium-a brief introduction to the parasites causing human malaria and their basic biology. J Physiol Anthropol. 2021;40(1):1. https://doi.org/10.1186/s40101-020-00251-9 PMid:33413683
Sebina I, Fogg LG, James KR, Soon MS, Akter J, Thomas BS, et al. IL-6 promotes CD4+ T-cell and B-cell activation during Plasmodium infection. Parasite Immunol. 2017;39(10):e12455. https://doi.org/10.1111/pim.12455 PMid:28748530
Kurup SP, Butler NS, Harty JT. T cell-mediated immunity to malaria. Nat Rev Immunol. 2019;19(7):457-71. https://doi.org/10.1038/s41577-019-0158-z PMid:30940932
Mohamedahmed KA, Abakar AD, Ahmed MO, Muktar MM, Nour BY. The role of TNF-α level as predictive diagnostic biomarker among children with severe falciparum malaria in endemic area in Sudan. Int J Acad Health Med Res. 2019;3(7):1-6.
Ortega-Pajares A, Rogerson SJ. The rough guide to monocytes in malaria infection. Front Immunol. 2018;9:2888. https://doi.org/10.3389/fimmu.2018.02888 PMid:30581439
Kany S, Vollrath JT, Relja B. Cytokines in inflammatory disease. Int J Mol Sci. 2019;20(23):6008. https://doi.org/10.3390/ijms20236008 PMid:31795299
Frimpong A, Amponsah J, Adjokatseh AS, Agyemang D, Bentum-Ennin L, Ofori EA, et al. Asymptomatic malaria infection is maintained by a balanced pro-and anti-inflammatory response. Front Microbiol. 2020;11:559255. https://doi.org/10.3389/fmicb.2020.559255 PMid:33281757
Ann A. Nutrition, food security and health. In: Kliegman R, editor. Nelson Textbook of Pediatrics. 20th ed. Philadelphia, PA: Elsevier Inc.; 2015. p. 295-306.
Oyegue-Liabagui SL, Bouopda-Tuedom AG, Kouna CL, Maghendji-Nzondo S, Nzoughe H, Tchitoula-Makaya N, et al. Pro-and anti-inflammatory cytokines in children with malaria in Franceville, Gabon. Am J Clin Exp Immunol. 2017;6(2):9-20. https://doi.org/10.1051/parasite/2016032 PMid:28337387
Farrington L, Vance H, Rek J, Prahl M, Jagannathan P, Katureebe A, et al. Both inflammatory and regulatory cytokine responses to malaria are blunted with increasing age in highly exposed children. Malar J. 2017;16(1):499. https://doi.org/10.1186/s12936-017-2148-6 PMid:29284469
Hanisch BR, Bangirana P, Opoka RO, Park GS, John CC. Thrombocytopenia may mediate disease severity in Plasmodium falciparum malaria through reduced transforming growth factor beta-1 regulation of proinflammatory and anti-inflammatory cytokines. Pediatr Infect Dis J. 2015;34(7):783-8. https://doi.org/10.1097/INF.0000000000000729 PMid:25886788
Kotepui KU, Kwankae w P, Masangkay FR, Mahittikorn A, Kotepui M. Transforming growth factor-β concerning malarial infection and severity: A systematic review and meta-analysis. Trop Med Infect Dis. 2022;7(10):299. https://doi.org/10.3390/tropicalmed7100299 PMid:36288040
Kurup SP, Obeng-Adjei N, Anthony SM, Traore B, Doumbo OK, Crompton PD, et al. Regulatory T cells impede acute and long- term immunity to blood-stage malaria through CTLA-4. Nat Med. 2017;23(10):1220-5. https://doi.org/10.1038/nm.4395 PMid:28892065