Botanical Larvicide from Jeruk Limau (Citrus amblycarpa) and Serai (Cymbopogon citratus) for Killing Aedes aegypti Larvae

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Refa Rahmaddiansyah
Eka Nofita
Sukarsi Rusti


BACKGROUND: Dengue hemorrhagic fever is caused by the dengue virus which is transmitted through the bite of the Aedes aegypti mosquito which breeds in densely populated areas with humid and warm climates. The use of anti-mosquito drugs in the form of burns, sprays, lotions, or other forms has been widely used, but these drugs are not completely profitable. Anticipatory efforts need to be made by utilizing natural materials so that they can be used effectively and safely for both humans and the environment.

AIM: Our study tries to find the effectivity of the infusion from Jeruk Limau (Citrus amblycarpa) and Serai for botanical larvicide against Aedes aegypti larvae.

METHODS: This research is a pure experimental study with a Post-test Only Control Group Design by making larvicide products from lime and lemongrass waste infusions, the results of which are analyzed descriptively and analytically. The research used two factors, namely treatment (P) using infusion products whereas control (K) using distilled water.

RESULTS: From the results of effectiveness tests on larvae, it was found that there was a reduction in live larvae until all of them died. Lime and lemongrass waste contain flavonoid compounds which are respiratory toxins in larvae. The combination of saponin and tannin as a stomach poison has the power to kill mosquito larvae. The abundant availability of materials, enriching research treasures on local potential, as well as a good level of public acceptance are the advantages of the product.

CONCLUSION: Apart from that, it is recommended to optimize the dosage and durability of the product so that it can be further patented as a concrete step in realizing the sustainable development agenda in the health sector.


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Rahmaddiansyah R, Nofita E, Rusti S. Botanical Larvicide from Jeruk Limau (Citrus amblycarpa) and Serai (Cymbopogon citratus) for Killing Aedes aegypti Larvae. SEE J Immunol [Internet]. 2024 Feb. 20 [cited 2024 Apr. 16];7:39-42. Available from:
Clinical Immunology


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